I've been experimenting with syncing audio and video in a precise way (something like http://www.youtube.com/watch?v=dBVA0kyXX4k#t=2m20s), and have found that in every output format except "MPEG Optimizer...", the sync is a little off... or at least different than the preview.
Through experimentation, I have found that you can be off by one frame and not really notice, but two frames and things look "amateurly" synced. Given that there is one frame leeway, I figured that rounding due to frame rate differences are accounted for, but this is not the case.
In some cases, I have also noticed transition and F/X changes, depending on the output format. For example, I have an image of a statue with "AutoSketch" applied. In the preview, everything looks wonderful. In HD MP4 output, the statue is drawn top to bottom, then the base of the statue disappears and is re-drawn. In DV and WMV format, the drawing is noticeably different, even though the resolution is the same.
What gives? If we can't count on the output to be like the preview, then all that time spent making a killer sync is just wasted
